Revista Española de Cardiología (English Edition) Revista Española de Cardiología (English Edition)
Rev Esp Cardiol. 2003;56:1085-92 - Vol. 56 Num.11

Anatomy of Cardiac Nodes and Atrioventricular Specialized Conduction System

Damián Sánchez-Quintana a, Siew Yen Ho b

a Departamento de Anatomía Humana. Facultad de Medicina. Universidad de Extremadura. Badajoz. España.
b Cardiac Morphology. National Heart and Lung Institute. Imperial College and Royal Brompton & Harefield NHS Trust. Londres. Reino Unido.


Catheter ablation. Sinoatrial node. Atrioventricular node. His bundle. Myocardium.


Concomitant with the development of catheter ablation techniques for the treatment of atrial arrhythmias, there has been renewed interest in the morphologic arrangement of the cardiac conduction system. The first descriptions of the anatomy of the nodes and atrioventricular conduction system appeared nearly 100 years ago. Since then the subject has been controversial, possibly because of the early researchers' imprecise knowledge of histology. The components and structure of the specific conduction system in humans are similar to those found in commonly used laboratory animals. The conduction system is composed of specialized myocytes. Its atrial components, the sinus node and the atrioventricular node, are in contact with atrial myocardium. The His bundle penetrates the right fibrous trigone, then divides into two specialized ventricular bundle branches (right and left), which also are surrounded by a fibrous sheath that separates the specialized myocytes from the ordinary myocardium. Only at the distal ramifications of the bundle branches do the fibrous sheaths disappear, allowing continuity with the ventricular myocardium. Knowledge of the specialized myocardium can help in the development of potentially useful therapies for some forms of cardiac arrhythmia.



The classic studies of Stannius1 in 1852 were the first to propose that cardiac conduction was myogenic. About a century ago it was shown that specialized muscular tissue was responsible for the initiation and spread of the heart beat. In 1906, Sunao Tawara2 confirmed the existence of a muscular bundle described by His3 back in 1893. Also in 1906, Keith and Flack4 confirmed the existence of the His-Tawara system. One year later they described the structure of the sinoatrial (SA) node.5

Although Purkinje6 was the first to describe specialized ventricular fibers, he was unaware of their importance in the structure of the heart, and it was Tawara2 who showed that the muscular bundle described by His was continuous with the ventricular Purkinje fibers.6 Tawara's studies have recently been translated into English,7 although the first translation of part of his work into this language was undertaken by Robb8 in his 1965 text book. These works are basic reading for all researchers who would study the cardiac conduction system (CS).


The muscular bundle connecting the atria to the ventricles was described by His3 as a «penetrating bundle.» However, His did not observe the histological continuation of this bundle in the right atrium with the atrioventricular (AV) node, the ventricles, or the ventricular Purkinje cells. It was Tawara2 who recognized this connection while working for his doctorate under the direction of Aschoff. Earlier, in 1893, both Kent9 and His3 had described muscular AV connections which were the cause of much confusion for many years.10 Later, it was observed that these connections were not to be found in healthy hearts, but in those that were diseased.

Following on from the findings of Kent and His, both clinical cardiologists and physiologists searched for the structure responsible for generating the cardiac impulse. It was suspected that this was situated in the area where the superior vena cava and the right atrium joined; under experimental conditions this is the last part of the heart to stop beating (the so-called ultimum moriens). In 1907, Keith and Flack5 distinguished the SA or sinus node in all the mammals they studied, including humans. Its constituent cells were believed to be the site of origin of the cardiac impulse.

The CS arises in the SA node, which is found in the upper anterior right atrium (Figure 1). The AV node is found in a lower, posterior position in the atrium. The CS extends from the AV node to the penetrating bundle of His and then divides into the left and right bundle branches which descend through the interventricular septum, enveloped in a connective tissue sheath that isolates them from the surrounding muscular tissue. Inside the myocardium they are continuous with the Purkinje network (Figure 1).

Fig. 1. Diagrammatic representation of the cardiac conduction system (red). The penetrating Bundle of His perforates the fibrous atrioventricular (AV) plane.

Morphological-macroscopic areas of interest

Several macroscopic areas of interest help to locate the cardiac CS. The SA node, which is sub-epicardial (Figure 2a), is wedged into the juncture between the musculature of the superior vena cava and that of the atrial appendage. Its base is opposite the terminal crest. The distance between the SA node and the epicardium is 0.3±0.1 mm.11 In about 10% of persons, the node does not extend towards the inferior vena cava but lies in a horseshoe shape around the lower part of the orifice of the superior vena cava.12 The AV node is found at the base of the atrial septum at the apex of a triangular area first illustrated by Koch.13 This triangle is situated on the endocardial surface of the right atrium (Figures 2b and c), is bordered anteriorly by the insertion of the septal leaflet of the tricuspid valve, and posteriorly by a fibrous tendon known as the tendon of Todaro. This tendon is the fibrous subendocardial continuation of the Eustachian valve, and inserts into the atrial musculature separating the orifice of the coronary sinus from the fossa ovale. The apex of this triangle is formed superiorly by the junction of the anterior and posterior borders mentioned above, corresponding to the central fibrous body (CFB) of the heart. The base of the triangle is formed by the orifice of the coronary sinus together with the vestibule of the right atrium supporting the septal leaflet of the tricuspid valve. This base is known to electrophysiologists as the septal isthmus, and it is here where radio-frequency ablation of the slow pathway is performed in patients with AV nodal reentrant tachycardia.14

Fig. 2. A: lateral epicardial view of the right atrium with the site of the SA node shown by the dashed pink line. B and C: endocardial views (normal and with transillumination) of the posterior and septal walls of the right atrium to show the oval fossa (OF) and limits of the triangle of Koch (dashed white lines), the tendon of Todazo (TT) and the insertion of the septal cusp of the tricuspid valve (TV). The vestibule (V) of the right atrium and the orifice of the coronary sinus (CS) form the lower limit. The location of the AV node is shown by an oval nodule (pink). D: left ventricular view to show the membranous septum (transillumination). This is the point of emergence of the bundle of His and its continuity with the right and left bundle branches. The left bundle branch (LBB) is marked with dashed white lines. A indicates aorta; AA, atrial appendage; VC, superior vena cava; RV, right ventricle; LV, left ventricle; MV, mitral valve.

The continuation of AV conduction occurs via the penetrating bundle of His, the only part of the conductive axis that perforates the CFB. The CFB is formed by the union of the connective tissue of the aortic and mitral heart valve leaflets with the septal leaflet of the tricuspid valve--the so-called right fibrous trigone--and the membranous part of the interventricular septum. In many mammalian hearts, the trigone is fibrous, but bovine hearts have a central mass of bone or cartilage (the os cordis). In contrast, the fibrous tissue of sperm whale CFB is very loose. The membranous portion or septum, which can range in length, is a good guide for locating the AV bundle of His. This appears above this membranous portion after crossing the right fibrous trigone (Figure 2d), and then divides into the left and right bundle branches. The right branch passes through the septal musculature at the base of the medial papillary muscle of the right ventricle. It then becomes a thin cord that penetrates deep into the septomarginal trabeculation or moderator band connecting the medial and anterior papillary muscles. The origin of the left branch lies below the commissure between the right and non-coronary cusps of the aortic valve; it then descends through the subendocardium of the interventricular septum (Figure 2d). Its path is sometimes visible owing to the shiny fibrous lamina that sheathes it. The proximal part of the left branch is much longer than that of the right. Occasionally a third branch called «dead-end tract»15 is seen in fetal or infant hearts, and this continues the bundle of His in an anterio-superior direction towards the root of the aorta.

Structure of the nodes and the atrioventricular conduction system

Studies in which the histological techniques employed were similar to those used by Tawara2 and later workers such as Davies16 and Truex et al17 (to mention just a few) have shown that the CS of humans is arranged in a manner quite like that of other mammals (with slight variations between species and between hearts). Tawara2 reported the separation of the specialized myocytes from the normal or working myocytes by a thin sheet of connective tissue visible under the light microscope, and on this the criteria proposed by Aschoff18 and Mönckeberg19 for histological identification of the specialized myocardium are based. Simply put, specialized myocytes stand out from working myocytes when viewed under the light microscope, and can be «followed» from one histological section to the next. In his monograph, Robb8 preferred to define the conductive tissue with the term «connecting» rather than «conducting» system, because histological preparations better define cell morphology than function. He also observed differences in the texture of the specialized myocardium depending on the freshness of autopsy material and the fixing and staining methods used. Tawara2 was aware of this and pointed out the heterogeneity of specialized myocyte morphology even in histological sections of the heart. Within a given species, the most obvious differences are related to the age of the individuals examined.20 In recent years different molecular and immunohistochemical markers have been used to locate the conductive tissue in embryonic hearts of humans and other mammals. However, no specific marker has been found that that can highlight this tissue in adult humans.

In the normal human heart, the SA and AV nodes do not meet the criteria of Aschoff and Mönckeberg18,19 because they are not electrically insulated from the surrounding myocardium by connective or fatty tissue. Rather, they enter into contact with atrial working fibers after a small area composed of transitional cells. In the SA node, Keith and Flack5 distinguished between the sinus and working cells. Tawara2, however, indicated the difficulties he encountered in differentiating AV node cells from those of the bundle of His. He therefore proposed that the difference between them was purely anatomical. On the basis of this definition, the portion of the CS completely sheathed by the CFB is termed the penetrating bundle or bundle of His (Figure 3a). The atrial portion from the proximal conduction system to the bundle of His is called the AV node (Figure 3b). This anatomical distinction is logical because the insulation of the penetrating bundle of His prevents it from making direct contact with the electrical activity of the afferent atrium. Any atrial activity must therefore be previously directed through the AV node.

Fig. 3. Sagittal histological sections of the sinoatrial (SA) node of the human (a;x10) and pig heart (b;x40) stained with the van Gieson method. Note the contact between sinus cells (SC) and working atrial cells (WAC). Sinus cells are characterized by being clearer and embedded in a greater amount of connective tissue (red). c: van Gieson-stained section of the mid-zone of the triangle of Koch. Note the shape of the compact AV node and the transitional cells (TC) in contact with the convex surface of the compact node. d: Masson's trichrome-stained section showing the penetrating bundle of His surrounded by fibrous tissue (green) from the CFB. SNA indicates sinus node artery; CFB, central fibrous body; TV, tricuspid valve.

The intrinsic function of the SA node is to be the source of the cardiac impulse. The SA node in humans is an arched or fusiform structure. Histologically it is composed of cells slightly smaller than normal working cells which are arranged in bundles. These mix together with no spatial order, stain weakly, and are embedded in a dense connective tissue matrix (Figures 3 a and b). With age, the amount of connective tissue increases with respect to the area occupied by the nodal cells.21 On the periphery of the node, specialized cells are mixed with those of the working myocardium (Figures 3a and b). In addition, multiple radiations or extensions interdigitating with the working atrial myocardium have been described. These penetrate intramyocardially into the terminal crest, and the superior and inferior vena cavae. The SA node is arranged around an artery known as the sinus node artery, which can run centrally or eccentrically inside the node. In 29% of human hearts this artery ramifies inside the node.11 The SA node is also intimately associated with the autonomic nervous system. It has been suggested that the majority of these nerve fibers are parasympathetic, the sympathetic fibers being concentrated around the node's blood vessels.23

The inherent function of the AV node is to delay the cardiac impulse. In humans, this node has a compact portion and an area of transitional cells. The former is semi-oval and lies over the CFB (Figure 3c). In the sections close to the base of the triangle of Koch, the compact part of the node divides into two extensions or prolongations. The artery vascularizing the AV node is usually found between these. The length of these extensions varies from one heart to another.24 The size of the transitional cells is intermediate between those of the AV node and the atrial working cells. They are surrounded by a greater quantity of connective cells than that covering the working cells, but they are not insulated from the adjacent myocardium. Rather, they form a kind of bridge between the working and nodal myocardium, and collect electrical information from the atrial walls, transmitting it to the AV node.

Controversy surrounds how the impulse from the SA node reaches the AV node. Some authors have suggested the existence of specialized tracts between them.25 Our studies do not support this idea but favor the hypothesis that the working muscle fibers themselves (and their geometric arrangement in the atrial walls) are responsible for conduction being more rapid in some areas of the atrium than in others.26

The AV node continues distally with the penetrating bundle of His (Figure 3d), although there are slight differences in terms of cellular arrangement between these two structures, including the arrangement of the bundle of His cells in a more parallel fashion. The explanation for this might be morphological: the bundle of His starts to be surrounded by the connective tissue of the CFB, thus becoming a conducting tract that takes information to the ventricles.

The AV node of the dog is smaller than that of humans, but has a longer penetrating bundle of His.27 Some authors28 interpret this to mean that a portion of the AV node of the dog lies within the CFB. In the rabbit, other authors29 describe part of the bundle of His as though it formed part of the AV node, but this is a mistake (Figures 4a-d). The most outstanding morphological difference between the AV node of the dog and those of the rabbit and humans is that the former is not covered by transitional cells. In rats (with a resting heart rate 10 times faster than that of dogs or humans), the AV node is proportionally comparable to that of the dog, but the CFB is smaller.

Fig. 4. This composite figure shows the atrioventricular (AV) node plus the bundle of His and its right and left bundle branches in the rabbit. Horizontal bar in b represents 1 mm (same for all images). Masson's trichrome staining. A indicates aorta; TT, tendon of Todaro; RV, right ventricle; LV, left ventricle; TV, tricuspid valve.

When the histological trajectory of the conduction system is followed towards the penetrating bundle of His, the latter is seen to turn towards the left in many human hearts, and emerge on the muscular crest of the interventricular septum. Surrounded by connective tissue from the CFB, the length of the bundle of His can vary before splitting into the left and right bundle branches. The former branch cascades over the left side of the interventricular septum (Figures 5a and c). The division of the bundle of His resembles a jockey squatting above the muscular crest of the interventricular septum (Figure 5a). However, on occasions it is deviated towards the left (Figure 5c). When this occurs, the right branch enters the interior of the septum musculature (Figure 5b), appearing in the right ventricle in association with the insertion of the medial papillary muscle.

Fig. 5. a: van Gieson-stained section showing the bundle of His (human heart) over the membranous portion (MP) of the interventricular septum. b: at its origin, the right branch in this heart is intramyocardial and is surrounded by connective tissue (blue) (Jones' trichrome stain, x20). c: the division of the bundle of His in this heart is displaced over the left side of the muscular crest of the interventricular septum, and descends longitudinally under the endocardium of the left ventricle (Jones´ trichrome, x5). d: note the covering connective sheath (blue) of the left branch (Jones' trichrome, x10). A indicates aorta; E, endocardium; TV, tricuspid valve.

Along their proximal courses, the right and left bundle branches are covered by a fibrous lamina (Figures 5b and d). As Tawara2 showed (Figure 6a), in humans the left branch is typically divided into three fascicles with extensive intercommunication. These fascicles become ramified in the ventricular apex, and extend to the interior of the two papillary muscles of the mitral valve, but also back along the ventricular walls toward the cardiac base. More distally, in the apex of the ventricles of the human heart, it becomes almost impossible to trace the ramifications of the Purkinje fibers since these lose their fibrous coat and look much like the working myocardium.

Fig. 6. a: diagram by Tawara showing the trifascicular arrangement of the left bundle branch in Humans. b and c: a fresh calf heart in which the right and left ventricles have been opened. Subendocardial injections of Indian ink reveal the right and left bundle branches and the Purkinje network. Note in B the three fascicles of the left bundle branch (arrows), and in C the moderator band (MB). d: section of a calf heart (van Gieson staining, x100) obtained after injecting Indian ink into the Purkinje network), which is enveloped at its origin by connective tissue (red). e: subendocardial arrangement of the Purkinje network in the left ventricle of a calf. Note the elliptical arrangement of the network and offshoots from the edges that penetrate the myocardium (arrows). f: dissection of the ventricular muscular fibers of an adult human heart. Note the difference in arrangement between the medial and deep layers of the left ventricle. A indicates aorta; PT, pulmonary trunk; LV, left ventricle; LV, left ventricle; TV, tricuspid valve.

Subendothelial injection of India ink is one of the methods used to observe these fibrous sheets and to demonstrate the subendocardial course of the right and left bundle branches and their ramifications in ungulate hearts (Figures 6b and d). Our studies on the hearts of sheep and calves show these to vary somewhat from human hearts. Calf hearts are more similar to human hearts in that the fascicles of the left bundle branch are usually three in number and originate in the upper part of the interventricular septum (Figure 6b). However, sheep hearts show only two fascicles, and these appear halfway down the length of the septal wall. In both sheep and calf hearts, small muscular trabeculae cross the ventricular cavity--the so-called «false tendon»--which inside them carry distal ramifications of the His branches towards the papillary muscles and the adjacent ventricular walls. On the right side of the heart, the moderator band of both the sheep and calf heart is more slender than that of humans, but inside it always contains an offshoot of the right bundle branch (Figure 6c).

In ungulate hearts the subendocardial Purkinje network is elliptical in arrangement, both in the left and right ventricle (Figure 6e). In addition, from its contour arise branches that penetrate the ventricular walls, leading to new branches or anastamoses with other branches (Figure 6e). However, intramural branches of the Purkinje network have not been demonstrated in the human heart.30

A controversial point regarding the Purkinje network is the existence of transitional cells between the working ventricular myocardium and the Purkinje fibers.31 The anatomical and immunohistochemical studies of Oosthoek et al,30 show that, in bovine hearts, there is a very small zone of transitional cells where the Purkinje fibers lose their connective tissue cover. However, such cells have not been observed in the sheep heart.32 When the Purkinje fibers lose their connective tissue cover, electrical impulses pass from the CS to the working myocytes of the ventricles. The spatial orientation of the working myofibrils in the ventricle walls determines the anisotropic nature of ventricular conduction (Figure 6f).


Although differences exist between species, the structure of the nodes, as well as that of the remainder of the human AV conduction system, is similar to that of commonly used laboratory animals. The SA node, the structure that generates the cardiac impulse, is situated at one extreme of the right atrium. Impulses from it travel posteriorly in the atrial walls through an intricate but precise spatial arrangement of working atrial fibers until reaching the end of the atrium. At this end, transitional cells of the AV node receive the impulse and delay it prior to its transmission via the bundle of His. The latter crosses the insulating fibrous plane between the atria and ventricles, and transmits the impulse via two branches (the right and left bundle branches) towards the corresponding ventricles. Each of these branches is insulated by a connective sheath of working ventricular myocytes. This arrangement allows contact between the specialized and working myocytes only at the distal ramifications of the bundle of His. In this way, the AV conduction system, largely described by Tawara2 nearly 100 years ago, is structured to impart order to the transmission of cardiac impulses. Knowing the structure and location of specific conductive tissue within the heart could help provide solutions to different disturbances in cardiac rhythm.

Correspondence: Dr. D. Sánchez-Quintana.
Departamento de Anatomía Humana. Facultad de Medicina.
Avda. Elvas, s/n. 06071 Badajoz. España.


1. Stannius H. Zwei Reihen physiologischer Versuche. 1. Versuche am Froschherzen. Archiv für Anatomie and Physiologie, 1852;p. 85.
2. Tawara S. Das Reizleitungssystem des Säugetierherzens. Jena: Gustav Fischer, 1906.
3. His W Jr. Die Thätigkeit des embryonalen Herezens und deren Bedeutung für die Lehre von der Herzbewegung bein Erwachsenen. Arb Med Klinik Leipzig 1893;1:14-49.
4. Keith A, Flack MW. The auriculo-ventricular bundle of the human heart. Lancet 1906;2:359-64.
5. Keith A, Flack M. The form and nature of the muscular connections between the primary divisions of the vertebrate heart. J Anat Physiol 1907;41:172-89.
6. Purkinje JE. Mikrosckopisch neurologische Beobachtungen. Archiv für Anatomie Physiologie und Wissenchafliche Medicin 1845;12:281.
7. Tawara S. The conduction system of the mammalian heart. Translated by Kozo Suma and Munehiro Shimada. London: Imperial College Press, 2000.
8. Robb JS. Comparative basic cardiology. New York: Grune & Stratton, 1965;p 349-63.
9. Kent AFS. Researches on the structure and function of the mammalian hearts. J Physiol 1893;14:233-54.
10. Anderson RH, Ho SY, Gillette PC, Becker AE. Mahaim, Kent and abnormal atrioventricular conduction. Cardiovasc Res 1996;31:480-91.
11. Chiu I, Hung CR, How SW, Chen MR. Is the sinus node visible grossly? A histological study of normal hearts. Int J Cardiol 1989;22:83-7.
12. Anderson KR, Ho SY, Anderson RH. Location and vascular supply of sinus node in human heart. Br Heart J 1979;41:28-32.
13. Koch W. Weiter mitteilungen uber den Sinusknoten der Herzens. Verhandlungen der Deutschen Pathologischen Gesellschaft 1909;13:85.
14. Olgin JE, Ursell PC, Kao AK, Lesh MD. Pathological findings following slow pathway ablation for AV nodal reentrant tachycardia. J Cardiovasc Electrophysiol 1996;7:625-31.
15. Kurosawa H, Becker AE. Dead-end tract of the conduction axis. Int J Cardiol 1985;7:13-8.
16. Davies F. Conducting system of the vertebrate heart. Br Heart J 1942;4:66-76.
17. Truex RC, Smythe MQ, Taylor MJ. Reconstruction of the human sinuatrial node. Anat Rec 1967;159:371-8.
18. Aschoff L. Referat über die Herzstörungen in ihren Bezienhungen zu den spezifischen Muskelsystemen des Herzens. Ver Dtsch Pathol Ges 1910;14:3-35.
19. Mönckeberg JC. Zur Entwicklungsgeschichte des Atrioventrikularsystems. Ver Dtsch Pathol Ges 1913;16:228-49.
20. Waki K, Kin JS, Becker AE. Morphology of the human atrioventricular node is age dependent: a feature of potential clinical significance. J Cardiovasc Electrophysiol 2000;11:1144-51.
21. Inoue S, Shinohara F, Niitani H, Gotoh K. A new method for the histological study of aging changes in the sinoatrial node. Jpn Heart J 1986;27:653-60.
22. Chuaqui B. Lupenpräparatorische Darstellung der Ausbreitungszüge des Sinusknotens. Virchows Arch Abt A Path Anat 1972;356:141-53.
23. Crick SJ, Wharton J, Sheppard MN, Royston D, Yacoub MH, Anderson RH, et al. Innervation of the human cardiac conduction system. A quantitative immunohistochemical and histochemical study. Circulation 1994;89:1697-708.
24. Inoue S, Becker AE. Posterior extensions of the human compact atrioventricular node. A neglected anatomic feature of potential clinical significance. Circulation 1998;97:188-93.
25. James TN. The connecting pathways between the sinus node and the A-V node and between the right and the left atrium in the human heart. Am Heart J 1963;66:498-508.
26. Sánchez-Quintana D, Davies DW, Ho SY, Oslizlok P, Anderson RH. Architecture of the atrial musculature in and around the triangle of Koch: its potential relevance to atrioventricular nodal reentry. J Cardiovasc Electrophysiol 1997;8:1396-407.
27. Ho SY, Kilpatrick L, Kanai T, Germroth PG, Thompson RP, Anderson RH. The architecture of the atrioventricular conduction axis in dog compared to man: its significance to ablation of the atrioventricular nodal approaches. J Cardiovasc Electrophysiol 1995;6:26-39.
28. Racker DK, Kadis AH. Proximal atrioventricular bundle, atrioventricular node, and distal atrioventricular bundle are distinct anatomic structures with unique histological characteristics and innervation. Circulation 2000;101:1049-59.
29. Anderson RH, Janse MJ, van Capelle FJ, Billette J, Becker AE, Durrer D. A combined morphologic and electrophysiologic study of the atrioventricular node of the rabbit heart. Circ Res 1974;35:909-22.
30. Oosthoek PW, Virágh S, Lamers WH, Moorman AFM. Immunohistochemical delineation of the conduction system II: the atrioventricular node and Purkinje fibres. Circ Res 1993;73:482-91.
31. Tranun-Jensen J, Wilde AA, Vermeulen JT, Janse MJ. Morphology of electrophysiologically identified junctions between Purkinje fibres and ventricular muscle in rabbit and pig hearts. Circ Res 1991;69:429-37.
32. Ansari A, Ho SY, Anderson RH. Distribution of the Purkinje fibres in the sheep heart. Anat Rec 1999;254:92-7.

1885-5857/© 2003 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved

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