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Vol. 74. Issue 12.
Pages 1013-1022 (December 2021)
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Vol. 74. Issue 12.
Pages 1013-1022 (December 2021)
Original article
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Coronary endothelial and microvascular function distal to polymer-free and endothelial cell-capturing drug-eluting stents. The randomized FUNCOMBO trial
Función endotelial y microvascular distal a stents farmacoactivos sin polímero y captadores de células endoteliales. Estudio aleatorizado FUNCOMBO
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Josep Gómez-Laraa,b,
Corresponding author
gomezjosep@hotmail.com

Corresponding author: Department of Interventional Cardiology, Hospital Universitari de Bellvitge, Carrer de la Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona, Spain.
, Loreto Oyarzabala,b, Salvatore Brugalettac,d, Neus Salvatellae,f, Rafael Romagueraa,b, Gerard Rouraa,b, Lara Fuentesa,b, Pedro Pérez Fuentesc,d, Luis Ortega-Pazc,d, José L. Ferreiroa,b, Luis Teruela,b, Montserrat Gracidaa,b, Beatriz Vaquerizoe,f, Manel Sabatéc,d, Josep Comín-Coleta,b, Joan-Antoni Gómez-Hospitala,b
a Departament de Cardiologia Intervencionista, Hospital Universitari de Bellvitge, L’Hospitalet de Llobregat, Barcelona, Spain
b Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), L’Hospitalet de Llobregat, Barcelona, Spain
c Departament de Cardiologia Intervencionista, Hospital Clínic i Provincial de Barcelona, Barcelona, Spain
d Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
e Departament de Cardiologia Intervencionista, Hospital del Mar, Barcelona, Spain
f Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain
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Benjamin Beska, Daniell Edward Raharjo, Vijay Kunadian
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Tables (5)
Table 1. Baseline clinical and procedural characteristics
Table 2. Quantitative coronary angiography analysis of the stent segment
Table 3. Distal coronary segment vasomotor test results
Table 4. Microcirculatory function results
Table 5. Optical coherence tomography findings
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Additional material (1)
Abstract
Introduction and objectives

The vasomotor function of new-generation drug-eluting stents designed to enhance stent healing and reendothelialization is unknown. This study aimed to compare the endothelial function of the infarct-related artery (IRA) treated with bioactive circulating endothelial progenitor cell-capturing sirolimus-eluting stents (COMBO) vs polymer-free biolimus-eluting stents (BioFreedom) in ST-segment elevation myocardial infarction patients at 6 months. Secondary objectives were to compare the microcirculatory function of the IRA and stent healing at 6 months.

Methods

Sixty patients were randomized to bioactive sirolimus-eluting stent vs polymer-free biolimus-eluting stents implantation. At 6 months, patients underwent coronary angiography with vasomotor, microcirculatory and optical coherence tomography examinations. Endothelial dysfunction of the distal coronary segment was defined as ≥ 4% vasoconstriction to intracoronary acetylcholine infusion.

Results

Endothelial dysfunction was similarly observed between groups (64.0% vs 62.5%, respectively; P=.913). Mean lumen diameter decreased by 16.0 ±20.2% vs 16.1 ±21.6% during acetylcholine infusion (P=.983). Microcirculatory function was similar in the 2 groups: coronary flow reserve was 3.23 ±1.77 vs 3.23±1.62 (P=.992) and the index of microcirculatory resistance was 24.8±16.8 vs 21.3±12.0 (P=.440). Optical coherence tomography findings were similar: uncovered struts (2.3% vs 3.2%; P=.466), malapposed struts (0.1% vs 0.3%; P=.519) and major evaginations (7.1% vs 5.6%; P=.708) were observed in few cases.

Conclusions

Endothelial dysfunction of the IRA was frequent and was similarly observed with new-generation drug-eluting stents designed to enhance stent reendothelialization at 6 months. Endothelial dysfunction was observed despite almost preserved microcirculatory function and complete stent coverage. Larger and clinically powered studies are needed to assess the role of residual endothelial dysfunction in ST-segment elevation myocardial infarction patients.

Registered in ClinicalTrials.gov: NCT04202172

Keywords:
Drug-eluting stents
Endothelial dysfunction
Optical coherence tomography
ST-segment elevation myocardial infarction
Abbreviations:
BES
BMS
DES
IRA
SES
STEMI
Resumen
Introducción y objetivos

Aún no se conoce la función endotelial de los nuevos stents farmacoactivos diseñados para promover el recubrimiento y la reendotelización. El objetivo principal es comparar la función endotelial de la arteria responsable del infarto (ARI) tratada con stents bioactivos liberadores de sirolimus captadores de células progenitoras endoteliales circulantes (SES; COMBO) frente a la tratada con stents sin polímero liberadores de biolimus (BES; Biofreedom), así como comparar la función microvascular de la ARI y el grado de cicatrización de ambos dispositivos a los 6 meses.

Métodos

Se aleatorizó a 60 pacientes con infarto agudo de miocardio con elevación del ST (IAMCEST) a tratamiento con SES o BES. Tras 6 meses, todos los pacientes se sometieron a pruebas vasomotora mediante acetilcolina y nitroglicerina y de función microvascular mediante técnicas de termodilución y exploración con tomografía de coherencia óptica (OCT). Una respuesta vasoconstrictora a la acetilcolina ≥ 4% se definió como disfunción endotelial.

Resultados

Ambos grupos presentaron similares porcentajes de disfunción endotelial (el 64,0 frente al 62,5%; p=0,913) y función microvascular. La reserva coronaria de flujo fue de 3,23±1,77 frente a 3,23±1,62 (p=0,992) y el índice de resistencia microvascular, 24,8±16,8 frente a 21,3±12,0 (p=0,440). Los hallazgos de la OCT fueron parecidos e indicaban una cicatrización avanzada: proporciones de struts sin recubrir (el 2,3 frente al 3,2%; p=0,466), con mala aposición (el 0,1 frente al 0,3%; p=0,519) y de evaginaciones coronarias mayores (el 7,1 frente al 5,6%; p=0,708).

Conclusiones

Tras 6 meses, los nuevos stents farmacoactivos presentaron con frecuencia parecidas disfunciones endoteliales de la ARI. La disfunción endotelial se observó a pesar de la adecuada función microvascular y la cicatrización avanzada.

Registrado en ClinicalTrials.gov: NCT04202172

Palabras clave:
Stents farmacoactivos
Disfunción endotelial
Tomografía de coherencia óptica
Infarto agudo de miocardio con elevación del segmento ST
Full Text
INTRODUCTION

Primary percutaneous coronary intervention is the preferred reperfusion strategy in patients with ST-segment elevation myocardial infarction (STEMI). Endothelial function of the infarct-related artery (IRA) is often impaired in the contiguous distal segment.1 Endothelial dysfunction after drug-eluting stent (DES) implantation has been associated with persistent angina and adverse clinical outcomes.2

New-generation DES aim to enhance stent healing and re-endothelialization. Bioactive sirolimus-eluting stents (SES) (COMBO, OrbusNeich, The Netherlands) combine an abluminal bioabsorbable sirolimus-coated polymer with an adluminal CD34+antibody layer designed to capture circulating endothelial progenitor cells. In a preclinical swine model, bioactive SES showed a larger degree of strut re-reendothelialization than durable polymer DES at 14 days.3 The polymer-free biolimus A9-eluting stent (BES) (BioFreedom, Biosensors, Switzerland) is designed to release the antiproliferative drug a few days after stent implantation.4 For this reason, BES are considered to have similar re-endothelialization to bare metal stents (BMS). However, the epicardial and microcirculatory vasomotor function of the IRA treated with new-generation DES designed to enhance stent reendothelialization is still unknown.

The primary objective of the present study was to describe and compare the endothelial function of the distal IRA segment treated with bioactive SES (COMBO) vs polymer-free BES (BioFreedom) at 6 months. Secondary objectives were to describe and to compare the microcirculatory function and stent healing of the 2 devices at 6 months.

METHODSStudy design and population

This study is an investigator-initiated, descriptive, proof of concept, pivotal, multicenter, randomized trial promoted by the Spanish Society of Cardiology and funded by Orbus Neich (The Netherlands). The funding source and the promoter of the study had no role in the study design, data management, data analysis, or final report.

The inclusion and exclusion criteria of the study are detailed in the methods of the supplementary data. In summary, all informed STEMI patients with suitable clinical and anatomical conditions for enrollment in the study were randomized 1:1 to bioactive SES (COMBO) vs polymer-free BES (BioFreedom). Patients were randomized if they had Thrombolysis in Myocardial Infarction ≥ 2 flow after wire crossing, predilatation, or thrombus aspiration according to the operator's criteria. Antiplatelet and antithrombotic therapy was left to the operator's criteria according to the standard procedures of each participating Institution. All patients included in the study were requested to undergo a new coronary angiogram, as per protocol, at 6 months. The study was performed according to the provisions of the Declaration of Helsinki, and the study protocol was approved by the ethics committee of each participating center. Written informed consent was obtained from all patients.

Six-month invasive coronary procedure

Patients were requested to stop all vasomotor drugs at least 24hours before coronary angiography. Vasomotor drugs were not allowed before the vasomotor test in case the radial approach was used.

The 6-month invasive protocol consisted of 3 parts. First, an epicardial vasomotor test of the IRA was performed to assess the endothelial-dependent and endothelial-independent responses of the distal coronary segment. Endothelial-dependent function was examined by intracoronary infusion of acetylcholine at incremental doses of 10-6 M and 10-4 M according to previous publications.5 Acetylcholine infusion was given via a microcatheter (Teleport, OrbusNeich, The Netherlands) at least 5mm proximal to the proximal stent edge. The endothelial-independent function was investigated by bolus injection of 200μg of intracoronary nitroglycerin via a guiding catheter. A detailed explanation of the vasomotor test can be found in the methods of the supplementary data.

Second, microcirculatory function assessment was performed with a dedicated intracoronary wire with pressure and temperature sensors (PressureWire X Guidewire, Abbott, United States). According to previous publications,5 the index of microcirculatory resistance, coronary flow reserve and fractional flow reserve were performed under intravenous adenosine infusion (140μg/kg/min).

Finally, optical coherence tomography (OCT) imaging was performed with a dedicated catheter (Dragonfly OPTIS, Abbott, United States) according to standard procedures.

Angiographic analysis

Angiographic analysis was performed by a core laboratory (BARCICORE-Lab, Barcelona, Spain) using specific software for quantitative coronary angiography analysis (CASS 5.9; Pie Medical BV, The Netherlands). The analysts were blinded to the study groups.

The vasomotor responses of the distal coronary segment to endothelial-dependent and independent stimuli were assessed taking into account the core laboratory variability for mean lumen diameter repeated measurements. The 2 standard deviation difference between quantitative angiographic measurements of matched coronary segments is 3.9%.5,6 Therefore, a vasoconstrictive response to low-dose or high-dose acetylcholine infusion (meaning endothelial dysfunction) was defined when ≥ 4% vasoconstriction was observed with respect to the baseline 6-month mean lumen diameter. The distal coronary segment was defined as the segment between the stent edge and up to 20 to 40mm according to natural landmarks. Assessment of vasomotor changes is shown in figure 1.

Figure 1.

Quantitative coronary angiography analysis of the distal segment. A, B, C, D: 6-month vasomotor test angiographic images at baseline, acetylcholine doses, and nitroglycerin. Stent edges are marked with yellow lines. E, F, G, H: quantitative coronary angiogram of matched distal segments between the different vasomotor drugs. The mean lumen diameter of matched segments is shown in each respective image. NTG, nitroglycerin; QCA, quantitative coronary angiography.

(0.66MB).
Optical coherence tomography analysis

OCT analysis was performed by a core-laboratory (BARCICORE-Lab, Barcelona, Spain) using specific software for analysis (LightLab Imaging, United States). Two blinded analysts were requested to assess the following qualitative OCT findings in the entire pullback (0.2mm intervals) according to a previous study7: the neointima pattern at the cross-section with largest neointima area, observation of cross-sections with a ratio of uncovered to total stent struts ≥ 30%, presence of major coronary evaginations and neoatherosclerotic plaques. Figure 2 shows the main OCT qualitative findings observed in the study. Quantitative OCT data were analyzed each 1mm according to standard core laboratory procedures.7 A detailed description of the quantitative OCT analysis is provided in the methods of the supplementary data.

Figure 2.

Main optical coherence tomography qualitative findings. A: absent neointima; B: homogeneous neointima; C: heterogeneous neointima; D: layered neointima; E: RUTTS (ratio of uncovered to total stent struts) ≥ 30%, uncovered struts are shown with *; F: major coronary evagination; G: incomplete stent apposition; H: fibro-lipidic neoatherosclerotic plaque.

(0.93MB).
Statistical analysis

This is a hypothesis-generating pivotal study. Therefore, there was no sample size calculation since there were no previous data regarding the endothelial function of new-generation DES. Categorical variables are presented as counts and percentages, and continuous variables as mean±standard deviation. Comparisons of categorical variables were estimated with the chi-square or Fisher exact tests, as appropriate. Comparisons of continuous variables between groups were evaluated with the Student t-test or nonparametric Mann-Whitney or Kruskal-Wallis tests, as appropriate. Comparisons of the same parameter at different time points (such as mean lumen diameter changes during the vasomotor test) were assessed with generalized linear modelling for repeated measures. OCT strut level analysis was performed considering the clustering nature of the OCT data with generalized estimation equations. All struts were classified into the following types: apposed and covered, apposed and uncovered, malapposed and covered and malapposed and uncovered. Each strut type was introduced into the model as a dependent variable using the binary logistic model. Each model was performed introducing stent type as covariate and patient identification as a subject variable. A 2-sided P value<.05 was considered statistically significant. Statistical analysis was performed with the SPSS software, version 20.0 (SPSS Inc, United States).

RESULTSPopulation

A total of 60 patients were included (31 bioactive SES COMBO and 29 polymer-free BES BioFreedom) in 3 institutions from November 2018 to September 2019. No clinical events or unscheduled angiographic follow-ups were documented at 6 months. Eight patients refused angiographic follow-up and 1 patient was excluded due to current chemotherapy treatment. Therefore, 51 patients (25 bioactive SES and 26 polymer-free BES) underwent invasive examination, as per protocol, at 6 months.

One patient had coronary disease progression (left main stenosis) and was excluded for further invasive examinations. Another patient had symptomatic paroxysmal atrial fibrillation during acetylcholine infusion and did not undergo a microcirculatory function test and OCT imaging. The flow chart of the study is shown in figure 3.

Figure 3.

Flow chart of the study. * 1 patient was diagnosed with colon cancer 1 month after the baseline procedure and was treated with chemotherapy during the angiographic follow-up period. This patient was excluded from invasive angiographic follow-up. BES, biolimus-eluting stent; NTG, nitroglycerin; OCT, optical coherence tomography; SES, sirolimus-eluting stent.

(0.33MB).
Baseline clinical and angiographic characteristics

Baseline clinical and procedural characteristics are shown in table 1. The main clinical characteristics were similar between groups. Most patients had complete occlusion of the culprit vessel (54.8% bioactive SES vs 48.3% polymer-free BES; P=.692). The most common IRA was the left anterior descending artery (48.4% vs 44.8%; P=.989).

Table 1.

Baseline clinical and procedural characteristics

  Bioactive SES (n=31)  Polymer-free BES (n=29)  P 
Age, y  57.2±9.7  57.1±9.0  .969 
Male sex  24 (77.4)  27 (93.1)  .089 
Body mass index  27.4±4.0  28.1±4.4  .502 
Smoking status      .951 
No  6 (19.4)  6 (20.7)   
Current smoker  21 (67.7)  20 (69.0)   
Former smoker  4 (12.9)  3 (10.3)   
Hypertension  10 (32.3)  14 (48.3)  .206 
Hypercholesterolemia  16 (51.6)  17 (58.6)  .586 
Diabetes mellitus  2 (6.5)  6 (20.7)  .105 
Insulin-treated diabetes mellitus  2 (6.9)  .137 
Previous PCI  1 (3.2)  .329 
Timing for primary PCI, min*
Onset chest pain – electrocardiogram  75 [44-200]  72 [50-150]  .709 
Onset chest pain – PCI  150 [127-270]  165 [130-250]  .742 
Number of diseased vessels      .653 
23 (74.2)  20 (69.0)   
8 (25.8)  9 (31.0)   
Culprit vessel      .989 
LAD  15 (48.4)  13 (44.8)   
LCX  5 (16.1)  6 (20.7)   
RCA  11 (35.5)  10 (34.5)   
TIMI-flow pretreatment      .692 
17 (54.8)  14 (48.3)   
4 (12.9)  2 (6.9)   
7 (22.6)  8 (27.6)   
3 (9.7)  5 (17.2)   
Predilatation  7 (22.6)  6 (20.7)  .859 
Thrombus aspiration  9 (29.0)  12 (41.4)  .316 
Number of study devices      .066 
31 (100)  26 (86.7)   
3 (10.3)   
Nominal study device diameter  3.3±0.5  3.3±0.4  .992 
Total study device length  19.8±4.9  21.0±5.5  .353 
Postdilatation  4 (12.9)  1 (3.4)  .185 
TIMI-flow posttreatment      .514 
1 (3.2)  2 (6.9)   
30 (96.8)  27 (93.1)   
ST-segment resolution, %  69.5±27.8  76.1±27.2  .406 
Ejection fraction, %  52.4±10.6  52.0±7.3  .885 

BES, biolimus-eluting stent; LAD, left anterior descending; LCX, left circumflex; PCI, percutaneous coronary intervention; RCA, right coronary artery; SES, sirolimus-eluting stent; TIMI, thrombolysis in myocardial infarction.

The data are presented as No (%) or mean±standard deviation.

*

PCI timings are expressed as median [interquartile range]

Quantitative coronary angiography results

The angiographic analysis of the stent segment are shown in table 2. Postprocedural results were similar in the 2 groups. At 6 months, lumen loss was similar between the groups (0.33±0.31mm vs 0.36±0.61mm, respectively; P=.814). Binary restenosis was observed in 8.0% vs 7.7%, respectively (P=.967).

Table 2.

Quantitative coronary angiography analysis of the stent segment

  Bioactive SES (n=25)  Polymer-free BES (n=26)  P 
In-stent analysis
Baseline (post-PCI)
Stent length, mm  18.37±4.52  20.10±4.96  .199 
Minimal lumen diameter, mm  2.69±0.39  2.70±0.40  .918 
Reference lumen diameter, mm  2.80±0.56  2.83±0.50  .878 
Diameter stenosis, %  2.24±13.54  3.54±12.78  .726 
Mean lumen diameter, mm  3.04±0.39  3.08±0.42  .732 
6-month follow-up (post-NTG)
Stent length, mm  17.76±4.43  20.10±4.94  .082 
Minimal lumen diameter, mm  2.36±0.53  2.34±0.64  .902 
Late lumen loss, mm  0.33±0.31  0.36±0.61  .814 
Reference lumen diameter, mm  2.81±0.55  2.71±0.58  .514 
Diameter stenosis, %  14.06±20.01  10.17±26.80  .559 
Binary restenosis, %  2 (8.0)  2 (7.7)  .967 
Mean lumen diameter, mm  2.77±0.44  2.82±0.39  .626 
In-segment analysis
Baseline (post-PCI)
Segment length, mm  27.36±4.74  28.96±5.67  .277 
Minimal lumen diameter, mm  2.21±0.43  2.31±0.42  .396 
Reference lumen diameter, mm  2.66±0.58  2.66±0.48  .967 
Diameter stenosis, %  15.60±12.94  12.48±11.78  .372 
Mean lumen diameter, mm  2.93±0.39  2.97±0.44  .664 
6-month follow-up (post-NTG)
Segment length, mm  26.52±5.17  28.67±5.22  .147 
Minimal lumen diameter, mm  2.08±0.49  2.03±0.59  .750 
Late lumen loss, mm  0.13±0.33  0.27±0.61  .313 
Reference lumen diameter, mm  2.67±0.45  2.73±0.39  .452 
Diameter stenosis, %  20.04±14.85  16.52±24.42  .536 
Binary restenosis, %  3 (12.0)  2 (7.7)  .605 
Mean lumen diameter, mm  2.74±0.41  2.78±0.38  .581 

BES, biolimus-eluting stent; NTG, nitroglycerin; PCI, percutaneous coronary intervention; SES, sirolimus-eluting stent.

Values are expressed as No. (%) or mean±standard deviation.

Vasomotor examination was performed in 49 patients (25 bioactive SES and 24 polymer-free BES). The endothelial-dependent and independent vasomotor responses at 6 months are shown in table 3. Both bioactive SES and polymer-free BES showed vasoconstriction to low dose (−8.3±20.1% vs7.6±14.2%; P=.890) and high dose (−16.0±20.2% vs16.1±21.6%; P=.983) of acetylcholine infusion. Endothelial dysfunction was frequent and was similarly observed in the 2 groups (64.0% vs 62.5%, respectively; P=.913). The mean lumen diameter changes of the distal coronary segment at 6 months is shown in figure 4.

Table 3.

Distal coronary segment vasomotor test results

  Stent type  Baseline  Ach M-6  Ach M-4  NTG  Pa  Pb 
Segment length, mmBioactive SES (n=25)  30.93±6.40  31.36±6.54  30.83±6.50  30.97±6.43  .992  .987 
Polymer-free BES (n=24)  30.92±8.52  30.87±9.07  31.67±8.39  30.76±8.15  .982   
Minimal lumen diameter, mmBioactive SES (n=25)  1.67±0.41  1.53±0.62  1.27±0.59  1.83±0.48  .003  .508 
Polymer-free BES (n=24)  1.59±0.33  1.42±0.52  1.19±0.54  1.79±0.39  <.001   
Reference lumen diameter, mmBioactive SES (n=25)  2.07±0.54  2.03±0.63  1.82±0.67  2.32±0.63  .050  .481 
Polymer-free BES (n=24)  2.08±0.45  1.86±0.47  1.66±0.55  2.21±0.54  .001   
Diameter stenosis, %Bioactive SES (n=25)  18.00±13.67  25.22±17.74  30.50±17.29  19.88±11.70  .021  .913 
Polymer-free BES (n=24)  22.89±12.09  24.88±17.48  29.42±20.30  17.80±12.40  .094   
Mean lumen diameter, mmBioactive SES (n=25)  2.18±0.47  2.00±0.65  1.84±0.65  2.38±0.52  .010  .562 
Polymer-free BES (n=24)  2.09±0.37  1.94±0.46  1.75±0.54  2.31±0.36  <.001   

Ach, acetylcholine; BES, biolimus-eluting stent; NTG, nitroglycerin; SES, sirolimus-eluting stent.

Values are expressed as mean±standard deviation.

a

Comparison of distal coronary segment lumen changes during the 6-month follow-up measurements within stent types using ANOVA test.

b

Comparison of distal coronary segment lumen changes during the 6-month follow-up measurements between stent types using generalized lineal model for repeated measures.

Figure 4.

Vasomotor test at 6 months. Mean lumen diameter changes to vasomotor test of the infarct-related artery distal segment at 6 months. BES, biolimus-eluting stent; SES, sirolimus-eluting stent.

(0.09MB).
Microcirculation function results

Microcirculatory function at 6 months is shown in table 4. Both bioactive SES and polymer-free BES had similar functional resting conditions. Hyperemic microcirculatory parameters were also similar between the groups and were within the normal reference values. Mean coronary flow reserve was 3.23±1.77 vs 3.23±1.62 (P=.992) and the index of microcirculatory resistance was 24.75±16.84 vs 21.30±11.98, respectively (P=.440).

Table 4.

Microcirculatory function results

  Bioactive SES (n=24)  Polymer-free BES (n=25)  P 
Baseline parameters
Mean aortic pressure, mmHg  83.60±14.64  86.00±14.25  .590 
Mean distal pressure, mmHg  79.00±15.61  80.13±15.79  .815 
Pd/Pa  0.94±0.05  0.93±0.09  .591 
Mean transit time, sec  1.10±0.59  0.86±0.40  .131 
Resting full-cycle ratio  0.91±0.07  0.91±0.13  .890 
Hyperemic parameters
Mean aortic pressure, mmHg  73.85±11.65  80.48±10.42  .058 
Mean distal pressure, mmHg  64.65±12.22  69.43±11.77  .191 
Pd/Pa (fractional flow reserve)  0.87±0.07  0.87±0.12  .908 
Mean transit time, sec  0.38±0.23  0.34±0.33  .663 
CFR  3.23±1.77  3.23±1.62  .992 
Normalized CFR  3.68±2.04  3.69±1.68  .978 
IMR  24.75±16.84  21.30±11.98  .440 
Corrected IMR  24.15±16.75  19.91±10.47  .335 
Resistive reserve ratio  4.08±2.44  4.76±3.87  .531 

BES, biolimus-eluting stent; CFR, coronary flow reserve; IMR, index microcirculatory resistance; Pd/Pa, distal pressure/aortic pressure; SES, sirolimus-eluting stent.

Values are expressed as mean±standard deviation.

Optical coherence tomography findings

OCT was performed in 48 patients (23 bioactive SES and 25 polymer-free BES). OCT findings at 6 months are shown in table 5. All qualitative and quantitative OCT parameters were similar between the 2 groups and demonstrated a high grade of stent healing at 6 months. As examples, the percentage of uncovered struts (2.3% vs 3.2%; P=.466), patients with >5% of uncovered struts (13.0% vs 20.0%; P=.518) and major coronary evaginations (7.1% vs 5.6%; P=.708) were observed in only few cases. In-stent neoatherosclerosis was observed in 8.7% vs 16.0%, respectively (P=.445).

Table 5.

Optical coherence tomography findings

  Bioactive SES (stent=23) (struts=4617)  Polymer-free BES (n=25) (struts=4803)  P
Qualitative data (lesion level)
Neointima pattern      .573 
Absent  3 (13.0)  4 (16.0)   
Homogeneous  13 (56.5)  17 (68.0)   
Heterogeneous  1 (4.3)   
Layered  6 (26.1)  4 (16.0)   
Stent coverage
RUTTS ≥ 30%  3 (13.0)  7 (28.0)  .202 
Uncovered struts ≥ 5%  3 (13.0)  5 (20.0)  .518 
Uncovered struts ≥ 10%  2 (8.7)  3 (12.0)  .708 
Major coronary evaginations  1 (7.1)  1 (5.6)  .913 
Malapposition
Any  1 (4.3)  3 (12.0)  .338 
Malapposed struts ≥ 5%  1 (4.0)  .708 
Neoatherosclerosis  2 (8.7)  4 (16.0)  .445 
Quantitative data (lesion level)
Stent length, mm  20.3±4.3  22.6±5.6  .118 
Reference lumen area, mm2  8.6±3.1  9.0±3.6  .621 
In-stent lumen area, mm2
Minimal  5.1±2.5  5.4±2.0  .587 
Mean  6.4±2.4  7.3±2.1  1.181 
Stent area, mm2
Minimal  6.9±2.1  7.4±2.1  .469 
Mean  7.9±2.2  8.8±2.5  .190 
Neointima area, mm2
Mean  1.5±0.7  1.5±1.0  .958 
Neointimal obstruction, %  20.9±12.9  17.4±9.3  .292 
Area stenosis, %  39.4±22.8  35.2±21.6  .521 
Quantitative data (strut level)
Strut type      .717 
Apposed and covered  4511 (97.7)  4646 (96.7)   
Apposed and uncovered  100 (2.2)  116 (3.6)   
Malapposed and uncovered  5 (0.1)  11 (0.3)   
Malapposed and covered  1 (0.0)  2 (0.1)   
Uncovered struts  105 (2.3)  155 (3.2)  .466 
Malapposed struts  6 (0.1)  14 (0.3)  .519 
Neointima thickness, μm  190.7±165.3  167.9±176.7  .501 

BES, biolimus-eluting stent; RUTTS, ratio of uncovered to total stent struts; SES, sirolimus-eluting stent.

Values are expressed as No. (%) or mean±standard deviation.

* P value of strut level data has been estimated with generalized estimating equations taking into account the clustering nature of the optical coherence tomography data.

DISCUSSION

The main findings of the present study are: a) both bioactive SES (COMBO) and polymer-free BES (BioFreedom) showed mainly impaired endothelial-dependent vasomotor function and preserved endothelial-independent function of the distal epicardial IRA at 6 months; b) the microcirculatory function of the IRA had an almost preserved response to hyperemia without differences between the study groups; c) both bioactive SES and polymer-free BES showed an advanced healing state, as assessed by OCT, at 6 months.

The coronary endothelium is the natural monolayer cell barrier between blood and arterial wall. According to pathology studies, stent implantation causes denudation of the endothelium and provokes an inflammatory response. At the very early phase after BMS implantation (< 30 days), inflammatory cell infiltration, platelet aggregation and fibrin deposition are normally observed.8 Simultaneously, smooth muscle cell migration accompanied by extracellular matrix deposition often surround and cover the stent struts. For this reason, as assessed by OCT, BMS exhibit most of the stent struts apparently covered at 30 days. However, stent re-endothelialization after BMS implantation occurs 3 to 4 months later by proliferation and migration of surrounding vascular endothelial cells and by the adhesion and maturation of circulating endothelial progenitor cells.9 Unfortunately, OCT is unable to assess stent re-endothelialization because of the limited image resolution.

The healing process of durable and bioresorbable polymer DES is temporarily and substantially different than that observed with BMS. The antiproliferative drug inhibits smooth muscle cells and vascular endothelial cell migration at the very early phase, delaying the stent healing process even at very long-term follow-up.8 Polymer-free DES are designed to enhance the stent healing process by a fast release of the antiproliferative drug (most of the drug is released in <48hours). Therefore, the healing process of polymer-free BES is similar to that observed with BMS. One study using polymer-free BES showed almost complete stent coverage, as assessed by OCT, at 4 months.4 Bioactive SES take a further step by aiming to adhere circulating endothelial progenitor cell to the endoluminal stent surface, while the abluminal surface inhibits smooth muscle cell proliferation. Preclinical studies demonstrated almost complete re-endothelialization of the inner surface of the stent at 14 days.3 According to several large controlled studies with elective angiographic follow-up between 6 and 13 months, current types of durable polymer and bioresorbable polymer DES show <0.20mm angiographic lumen loss. In contrast, DES aimed to enhance stent healing, such as polymer-free BES and bioactive SES, show lumen loss >0.20mm. Although further investigations with large numbers of patients are required, DES aimed to enhance stent re-endothelialization seem to show a larger neointima response and restenosis than current iterations of durable polymer and bioresorbable polymer DES. Table 1 of the supplementary data summarizes the in-stent results of most of the studies using current generation DES with angiographic follow-up.

Several randomized trials have shown differences regarding the endothelial function of different stent types in non-STEMI patients. It is commonly assumed that BMS mostly preserve the normal endothelial function of the distal coronary segment (vasodilatation) when the stent has fulfilled the healing process (approximately at 6 months). Mean lumen diameter changes to endothelial-dependent stimuli of the distal coronary segment treated with BMS are reported to be between2.5% to+8.6%.10–12 However, the small numbers of patients, use of different vasomotor tests (such as rapid pacing, supine exercise, or acetylcholine infusion) and different methods used for quantitative coronary angiography analysis warrant careful interpretation of these data. First-generation durable polymer DES are commonly accepted to lead to the worst endothelial function (vasoconstriction between 23.6% to 3.4%)10,11 and second-generation durable polymer DES (vasoconstriction between 9.4% to 3.1%) and bioresorbable polymer DES (vasoconstriction around 8.6%) show a certain degree of endothelial dysfunction.1,6,12,13

Endothelial dysfunction seems more intense in STEMI patients.14 First, STEMI patients show systemic inflammation and microvascular dysfunction of several organs and coronary vessels affecting the normal epicardial endothelial function.2 Second, stent implantation modifies the vessel geometry and endothelial shear stress forces, especially in the contiguous stent segments. Coronary segments with low endothelial shear stress, such as stent edge segments, show larger degree of endothelial dysfunction than segments with normal or high endothelial shear stress.15 Finally, stent implantation denudates the coronary endothelium and consequently, endothelial dysfunction is generally observed in distal coronary segments immediately after stent implantation.16 DES are designed to delay stent healing and reendothelization and are associated with a larger amount of malapposed and protruding stent struts than BMS. Malapposed and protruding stent struts cause flow disturbances similar to those observed in segments with low endothelial shear stress.15 Notably, STEMI lesions treated with DES show worse stent healing than non-STEMI lesions.17,18 In addition, the direct drug action of current DES, inflammatory reaction to different stent polymers and the degree of stent re-endothelialization have been pointed out as potential mechanisms of endothelial dysfunction.15

According to the few studies performed of endothelial function in STEMI patients, distal segments of the IRA treated with BMS showed 7.9% vasoconstriction to intracoronary acetylcholine at 6 months19; bioresorbable polymer SES (Orsiro, Biotronik, Switzerland) showed 18.1±15.4% vasoconstriction at 1 year20; and durable polymer everolimus-eluting stent (XIENCE, Abbott, United States) showed 8.7±14.8% vasoconstriction at 3 years.5 Therefore, taking into account the limitations of comparing different studies with different angiographic follow-ups, bioactive SES (16.0±20.2% vasoconstriction) and polymer-free BES (16.1±21.6% vasoconstriction) seem to have a similar vasomotor response at 6 months as bioresorbable polymer SES at 1 year, but worse endothelial function than durable polymer everolimus-eluting stent at 3 years. The endothelial function of all 4 DES in STEMI patients is summarized in table 2 of the supplementary data.

Limitations

First, the present study has no sample size calculation. Therefore, all comparisons between devices are merely hypothesis generating. Second, this study recruited <10% of patients undergoing primary PCI during the study period and therefore the sample is not representative of an all-comers STEMI population. Third, vasomotor examination was performed at 6 months. Although both study devices have theoretically fulfilled the healing process according to preclinical studies, it is possible that human models may have slower healing and therefore the endothelial function of both devices could be better at longer follow-up. Finally, due to the methodology used in the present study, epicardial and microvascular vasospastic angina were not evaluated. To prevent complications related to provocative vasospastic tests, such as occlusion of proximal coronary segments, it was decided to selectively infuse intracoronary acetylcholine via a microcatheter.

CONCLUSIONS

IRA treated either with bioactive SES (COMBO) or polymer-free BES (BioFreedom) showed a similar epicardial distal endothelial vasomotor response to acetylcholine infusion and similar microcirculatory response to hyperemia at 6 months. Endothelial dysfunction was frequently observed despite preserved functional microcirculatory parameters of the IRA and almost complete stent coverage, as assessed by OCT at 6 months. Larger studies are needed to assess the role of endothelial dysfunction in STEMI patients.

FUNDING

This study was funded by OrbusNeich.

CONFLICTS OF INTEREST

J. Gómez-Lara received fees from BARCICORE-Lab. S. Brugaletta reports consultant fees from Boston Scientific and iVascular. M. Sabaté reports consultant fees from Abbott Vascular and iVascular. The other authors declare no conflicts of interest.

WHAT IS KNOWN ABOUT THE TOPIC?

  • -

    Distal segments to coronary stents show a different vasomotor response to endothelial-dependent stimuli. In general, BMS show better endothelial function than DES, which has been attributed to the better stent healing and re-endothelialization of BMS. Moreover, STEMI patients show worse stent healing of current DES than stents implanted in other clinical scenarios. New-generation DES, such as polymer-free and bioactive DES, are designed with the aim of enhancing stent coverage and re-endothelialization. However, the endothelial function of distal coronary segments treated with those stents in STEMI patients is largely unknown.

WHAT DOES THIS STUDY ADD?

  • -

    This is the first study investigating the coronary function of new stent technologies aiming to promote stent re-endothelialization in STEMI patients. Although minor differences between stent technologies can be hypothesized, the endothelial function observed in STEMI patients was severely impaired and may have multiple causes. Moreover, endothelial dysfunction was observed despite optimal stent healing and microvascular function. Further investigations are required to address the role of stent-related endothelial dysfunction in STEMI patients.

APPENDIX. SUPPLEMENTARY DATA

Supplementary data associated with this article can be found in the online version available at https://doi.org/10.1016/j.rec.2021.01.007

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