INTRODUCTION
Stretching changes the electrophysiological properties of both atrial and ventricular cardiac tissue by activating and opening stretch-sensitive channels, and causing alterations in the cellular ion currents.1 Because there is a large variety of mechanosensitive ion channels, an agent that can block all these channels has still not been identified. The aminoglycoside antibiotic streptomycin, although it is not specific, has been reported to block stretch-sensitive channels and reduce the development of arrhythmias induced by mechanical causes.2,3 Myocardial activation during ventricular fibrillation (VF) depends on electrophysiological properties, and for this reason stretching also changes the characteristics of activation during arrhythmia.4 The mechanisms producing these changes have not been clearly established, however. The present study investigates whether stretch-induced changes in the myocardial activation pattern during VF are counteracted by streptomycin.
METHODS
Experiment Preparation
The experiments were performed following the regulations set down in Spanish Royal Decree 1201/2005 of October 10, regarding the use of animals for scientific purposes.
Sixteen isolated, perfused, rabbit heart preparations were used, following previously described methods.5,6 An L-shaped device was inserted in the left ventricular cavity to produce local stretching of the left ventricular free wall (Figure 1). Recordings were obtained with 2 multiple electrodes placed in the epicardium of the left ventricle, 1 in the area submitted to local stretching (SA) and another in an unaltered area (NSA), with 121 and 119 unipolar electrodes, respectively.
Figure 1. Schematic diagram of the device used to produce local stretching, with epicardial multielectrodes in the study areas. LV (ANT) indicates anterior left ventricle; LV (PST), posterior left ventricle; SA, stretched area; NSA, nonstretched area.
Two series of experiments were performed following the same protocol (baseline-stretching-post-stretch), the only difference being the absence (series A, n=8) or presence (series B, n=8) of streptomycin (200 μmol) in the perfusion fluid of the heart since the start of the preparation.
Data Analyzed
Ventricular fibrillation was induced by overstimulation, perfusion was maintained, and the following parameters were determined in both series: the dominant frequency (DFr) during VF (Welch method5,6) (Figure 2), the interval between successive ventricular activations (VV interval), and the functional refractory period. These parameters were determined before (5-min period), during (10 min), and after (10 min) local ventricular wall stretching (longitudinal increase of 12%).
Figure 2. Power spectra of ventricular fibrillation in the altered area obtained from 1 experiment in each series at baseline and during stretching. DFr indicates dominant frequency (Hz); PSD, power spectrum density; NU, normalized units.
Statistical Calculations
The data are presented as the mean (standard deviation). Comparisons were performed using a general linear model with repeated measures, considering the phases of the experiment as a within-subject factor and the use of streptomycin or not as a between-subject factor, and applying the Bonferroni test as the post hoc test (P<.05, significant differences).
RESULTS
Effect of Streptomycin on Stretch-Induced DFr Changes
In series A, DFr was significantly increased in the SA (P=.002), but not in the NSA (general analysis, within-subject comparison test, factor phases of the experiment). Differences between the 2 heart areas were significant from minutes 2 to 6 of stretching (Figure 3).
Figure 3. Dominant frequency during ventricular fibrillation (mean [standard deviation]) obtained at 1-minute intervals in the stretched and nonstretched sreas of the heart in series A (above) and series B (below). E1 to E10 indicates stretching; DFr, dominant frequency (Hz); P1 to P10, post-stretch; SA, stretched area; NSA, nonstretched area. *SA versus NSA, P<.05.
In series B, the influence of the experiment phase factor was also significant in the SA (P=.001) and not in the NSA. Differences between the 2 areas were significant from minutes 2 to 4 of stretching.
Comparison between the 2 series showed that the DFr was lower in series B (streptomycin) than in series A in both areas of the heart (general analysis, between-subject differences: NSA, P=.027; SA, P=.016).
Effects of Streptomycin on Stretch-Induced VV Interval Changes during Ventricular Fibrillation
In series A, VV intervals in the SA were shorter during stretching with respect to baseline values (P=.04) and post-stretch values (P=.018), whereas in the NSA there were no significant changes (within-subject comparison, experiment phase factor) (Table 1).
In series B, VV intervals in the SA during stretching were also shorter relative to baseline (P=.002) and following stretching (P=.004) (within-subject comparison, experiment phase factor).
Comparison between the 2 series showed longer VV intervals in series B (streptomycin) than in series A in both heart areas (between-subject differences: NSA, P=.004; SA, P=.001).
Effects of Streptomycin on Stretch-Induced Changes in Electrophysiological Parameters
In series A, refractory periods during VF were also shorter in the SA during stretching (P=.026 vs baseline and P=.045 vs post-stretch values), whereas no changes were seen in the NSA (Table 2).
In series B, refractory periods were decreased in the SA during stretching (P=.01 vs baseline P=.001 vs post-stretch values) and the NSA showed no variations.
With the action of streptomycin (series B), values were higher in the NSA and differences did not reach statistical significance in the SA (between-subject differences: NSA, P=.047; SA, P=.075).
DISCUSSION
The main finding of this study is that streptomycin decreased the effects of myocardial stretching during VF, and had a slowing action on VF both during and in the absence of stretching. The results were obtained with high drug concentrations, well above the therapeutic dose in humans. The concentrations used were those described to avoid the changes produced by stretching.3
Garnier et al7 found that streptomycin inhibited the increased intracellular calcium concentration induced by stretching in ventricular myocytes; hence the finding of reductions in these concentrations would be one of the reasons for the decrease in stretch-induced arrhythmias in the presence of streptomycin. These authors reported that streptomycin had no effect on L-type calcium channels. Moreover, Eckard et al3 did not observe the results obtained with streptomycin when using verapamil as a specific calcium blocker, thereby demonstrating that the effects of streptomycin are produced by blocking the mechanosensitive ion channels. The present study has contributed to this research with data showing the action of streptomycin on myocardium that is not subjected to any manipulation: in addition to attenuating the effects of stretching, streptomycin had a slowing effect on VF in the absence of stretching. The electrophysiological effects inherent to streptomycin may explain the VF changes observed in the present study in the control situation, in the area that was not altered by stretching.
The capability of streptomycin to inhibit triphosphate inositol production may also be implicated in its possible antiarrhythmic effect. Du et al8 observed that different drug compounds, among them streptomycin and gentamicin, inhibit triphosphate inositol release during reperfusion, and that intravenous gentamicin can even suppress the onset of arrhythmias. This is a parallel mechanism, in which adenosine triphosphate is likely implicated.
Bauty et al9 and Sung et al10 have indicated that other mechanisms in addition to activation of stretch-sensitive channels may come into play during stretching. This fact might be related to the persistence of stretch-induced alterations in VF, despite the use of high concentrations of streptomycin.
In conclusion, streptomycin attenuated the electrophysiological changes and alterations in myocardial activation during VF produced by acute stretching and had a direct effect on the refractoriness and activation frequency.
ACKNOWLEDGEMENTS
The authors thank César Avellaneda and Camilo Gómez for their help in preparing the electrodes and stretching device, and Rosa Cibrián for performing the statistical analysis.
This study has been funded by grants from the Spanish Ministry of Health (FIS PI06/0758 and RETIC RD06/0003/0010 REDINSCOR) and the Spanish Society of Cardiology (SEC 2004).
Correspondence: Dr. F.J. Chorro Gascó.
Servicio de Cardiología. Hospital Clínico Universitario.
Avda. Blasco Ibáñez, 17. 46010 Valencia. España.
E-mail: Francisco. J. Chorro@uv.es
Received October 16, 2006.
Accepted for publication September 12, 2007.